Functional Protein Microarrays in Drug DiscoveryPaul F. Predki CRC Press, 30 mars 2007 - 480 pages An ideal text for biotechnologists, protein chemists, and biochemists, Functional Protein Microarrays in Drug Discovery explores all aspects of functional protein microarrays, including basic principles, methods, and applications. The book discusses the generation of functional protein content and describes both standard and state-of-the-art fabrication methods. It reviews current and next generation approaches to assay detection and presents a wide range of applications, from biomolecular interaction discovery and characterization to immune response profiling. The book also addresses several fundamental computational issues, bioinformatics and data analysis. |
Table des matières
| 3 | |
Chapter 2 Protein Expression for MicroArrays | 23 |
CellFree Protein Expression | 39 |
Fabrication of Functional Protein Microarrays | 51 |
Chapter 4 The Critical Role of Surface Chemistry in Protein Microarrays | 53 |
Chapter 5 Fabrication of SolGel Derived Protein Microarrays for Diagnostics and Screening | 73 |
Chapter 6 Printing and QC of Functional Protein Microarrays | 99 |
Chapter 7 Protein Engineering for Surface Attachment | 115 |
Rapid Identification of 1433 Protein Binding Partners | 239 |
Chapter 14 A Combined Force of Chemical Genetics and Protein Microarrays | 261 |
Chapter 15 Antibody Profiling for Protein Drug Development and Clinical Development | 275 |
Chapter 16 Humoral Response Profiling Using Protein Microarrays | 301 |
Chapter 17 DNA Interactions with Arrayed Proteins | 313 |
Chapter 18 G Protein8211Coupled Receptor Microarrays for Drug Discovery | 333 |
Chapter 19 Kinase Substrate Identification Using Yeast Protein Microarrays | 351 |
Bioinformatics Data Analysis | 361 |
Chapter 8 Protein In Situ Arrays through CellFree Protein Synthesis | 133 |
Detection Methods for Protein Microarrays | 145 |
Chapter 9 Fluorescent Detection Methods for Protein Microarrays | 147 |
Chapter 10 Functional Analysis of Protein Interactions Using Surface Plasmon ResonanceBased Microarrays | 181 |
At the Intersection of Protein Microarrays and Mass Spectrometry | 199 |
Chapter 12 HighResolution Label Free Detection Applied to Protein Microarray Research | 217 |
Applications of Functional Protein Microarrays | 237 |
Chapter 20 Protein Microarray Image Analysis | 363 |
Chapter 21 The Analysis of Protein Arrays | 381 |
Chapter 22 Evaluating Precision and Recall in Functional Protein Arrays | 403 |
Chapter 23 Visualization of Protein Microarray Data | 415 |
| 443 | |
Back cover | 461 |
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Expressions et termes fréquents
activity affinity Alexa Fluor amino acid Anal analysis antibody antigen applications approach assays autoantibodies autoimmune baculovirus binding Biochem Biol biological biosensor Biotechnol biotherapeutic biotin biotinylated buffer cancer cell cell-free Chem chemical chemistry chip cloning coli complex covalent databases detection DNA microarray drug dyes enzyme epitope Figure fluorescent functional protein arrays functional protein microarrays gel element gene genetic genome GPCR high-throughput human identified immobilized proteins incubated inhibitors Invitrogen labeled large number ligand lysate membrane method molecular motifs normalization optimal pathway peptide phage phosphorylation pixel posttranslational modifications probe production profiling protein arrays protein expression protein interactions protein kinase protein microarrays protein molecules protein–DNA protein–protein proteome purification reaction reagents receptor recombinant proteins samples screening sensor sequence signal slides small molecule sol-gel solution specific spots strategy substrates surface surface plasmon resonance synthesis target protein therapeutic transcription vitro vivo yeast